Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen . Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of .
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http://dx.doi.org/10.21769/BioProtoc.2289 | DOI Listing |
Appl Environ Microbiol
January 2025
Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
In Sweden, reforestation of managed forests relies predominantly on planting nursery-produced tree seedlings. However, the intense production using containerized cultivation systems (e.g.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
School of Life Sciences and Health, Huzhou College, Huzhou, Zhejiang, China.
subsp. () possesses a -specific uter embrane rotein XAC1347 (OMP) that exerts a role in the expression of the type III secretion system for pathogenicity. In this study, we reported that OMP was required for salt stress tolerance and cell membrane integrity, as well as the expression of the genes for the production of extracellular polysaccharides.
View Article and Find Full Text PDFMicrob Genom
January 2025
Departamento de Bioqumica, Instituto de Qumica, Universidade de So Paulo, So Paulo, SP, Brazil.
The São Paulo state citrus belt in Brazil is a major citrus production region. Since at least 1957, citrus plantations in this region have been affected by citrus canker, an economically damaging disease caused by subsp. ().
View Article and Find Full Text PDFEnviron Microbiol Rep
February 2025
Department of Systematic and Environmental Botany, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
The fungus Beauveria felina is often classified as one of the so-called good biocontrol agents. However, no information is available about the growth of this entomopathogenic fungus in the presence of other endophytic fungi, which are usually found in plant tissues. Effects of fungal interactions vary from inhibiting the activity of a biocontrol agent to stimulating its effect on the targeted pathogen.
View Article and Find Full Text PDFJ Integr Plant Biol
January 2025
Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City & Southwest University, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
The development of rapeseed with high resistance against the pathogen Sclerotinia sclerotiorum is impeded by the lack of effective resistance resources within host species. Unraveling the molecular basis of nonhost resistance (NHR) holds substantial value for resistance improvement in crops. In the present study, small RNA sequencing and transcriptome sequencing were carried out between rice (a nonhost species of S.
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