N-doped MoS-nanoflowers as peroxidase-like nanozymes for total antioxidant capacity assay.

Total Antioxidant Capacity (TAC) Assay plays an important role in evaluating the quality of antioxidant food and monitoring the oxidative stress level of human body. It is mainly achieved by measuring the contents of antioxidants such as AA, L-Cys and GSH, while TAC can be detected by using peroxidase-like activity of artificial nanoenzyme materials. In this work, the N-Doped, defect-rich N-MoSNFs nano-materials were used to build the nano enzyme, which has strong stability and high peroxidase-like activity. HO was detected because it can be catalyzed to generate the intermediate ·OH and make TMB appears blue. However, when HO, AA, L-Cys and GSH coexist in solution, due to the oxidation resistance of AA, L-Cys and GSH, they can competitively react with ·OH in solution or reduce TMB in oxidation state (oxTMB), which reduces the characteristic absorption of oxTMB, indirectly achieves the purpose of detecting AA, L-Cys and GSH, and finally realizes the determination of TAC, even in actual serum and saliva samples. At the same time, the N-MoS NFs/NH-MIL-53(Al)+OPD system is further constructed. Based on the fluorescence resonance energy transfer (FRET) between NH-MIL-53(Al) and oxidized OPD (oxOPD), the purpose of detecting TAC by fluorescence method was realized.