Rose petals-like Bi semimetal embedded on the zeolitic imidazolate frameworks based-immunochromatographic strip to sensitively detect acetamiprid.

Ultrasensitive and facile detection of Acetamiprid (ACE) is of exceptional significance to assess the environmental and biological pollution. In this study, an advanced Bi semimetal/Zeolitic imidazolate frameworks hybrid material-based immunochromatographic strip (Bi/ZIF HM-ICS) sensor was developed for the sensitive detection of ACE. The novel Bi/ZIF HM was prepared through one-pot hydrothermal reduction of Bi nanoparticles on ZIF, which was selected as a signal tag taking advantages of its excellent color intensity, strong affinity with monoclonal antibodies (mAbs), and favorable biocompatibility. Bi/ZIF HM could not only improve the utilization efficiency of mAbs but also boost the sensing performance. Under optimal conditions, the limit of detection (LOD) of the Bi/ZIF HM-ICS was 4.68 pg/mL with the linear range from 0.01 ng/mL to 6 ng/mL, which was 98-fold lower than that of traditional gold nanoparticles-based ICS (0.457 ng/mL), and the recoveries of the Bi/ZIF HM-ICS ranged from 80.27% to 118.52% with the relative standard deviation (RSD) below 3.67% in pear, apple, tomato, and cucumber. Overall, the practical application of the Bi/ZIF HM-ICS in complicated samples was realized for detecting pesticide residue, and expanding its application scope in monitoring environment.