AI Article Synopsis
- Sulfonylureas (SUs) are anti-diabetic drugs used for treating type 2 diabetes, but they are being illegally added to herbal teas for enhanced effectiveness.
- A new monoclonal antibody (anti-SUs mAb) was developed to recognize nine different SUs and was used to create a colloidal gold lateral flow immunoassay (CG-LFIA) for detection.
- The CG-LFIA showed high sensitivity and specificity in detecting SUs, making it a potential tool for on-site monitoring of illegal adulteration in herbal tea products.
Article Abstract
Sulfonylureas (SUs) are a series of anti-diabetic drugs widely used for type 2 diabetes mellitus for clinic treat. However, it is often illegally adulterated in multi-herbal tea to improve the claimed anti-diabetic activity in recent years. In this study, a novel hapten was rationally designed, and a broad-specific monoclonal antibody (anti-SUs mAb) recognizing nine SUs was developed. This mAb was used to develop a colloidal gold lateral flow immunochromatographic assay (CG-LFIA). The anti-SUs mAb demonstrated half inhibitory concentration (IC) ranged from 0.15 ng/mL to 3.25 ng/ mL for nine SUs by ELISA. The cut-off value of developed CG-LFIA for nine SUs was from 3 to 100 ng/ mL for the spiked samples. LC-MS/MS confirmed the reliability of the new CG-LFIA. The results indicated that the proposed CG-LFIA could be an ideal method in on-site screening surveillance assay for SUs illegally adulterated in multi-herbal tea products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.foodchem.2021.131055 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Broad-specific immunochromatography for simultaneous detection of various sulfonylureas in adulterated multi-herbal tea.
Food Chem
February 2022
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address:
Article Synopsis
- Sulfonylureas (SUs) are anti-diabetic drugs used for treating type 2 diabetes, but they are being illegally added to herbal teas for enhanced effectiveness.
- A new monoclonal antibody (anti-SUs mAb) was developed to recognize nine different SUs and was used to create a colloidal gold lateral flow immunoassay (CG-LFIA) for detection.
- The CG-LFIA showed high sensitivity and specificity in detecting SUs, making it a potential tool for on-site monitoring of illegal adulteration in herbal tea products.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!