Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554525 | PMC |
http://dx.doi.org/10.1016/j.niox.2021.09.004 | DOI Listing |
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