Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Nitric Oxide

Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA. Electronic address:

Published: December 2021

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554525PMC
http://dx.doi.org/10.1016/j.niox.2021.09.004DOI Listing

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