Corona protein impacts on alternating current biosusceptometry signal and circulation times of differently coated MnFeO nanoparticles.

We evaluated the impacts of corona protein (CP) formation on the alternating current biosusceptometry (ACB) signal intensity and circulation times of three differently coated magnetic nanoparticles (MNP): bare, citrate-coated and bovine serum albumin-coated MNPs. We employed the ACB system, gel electrophoresis and mass spectrometry analysis. Higher CP formation led to a greater reduction in the ACB signal intensity and circulation time. We found fewer proteins forming the CP for the bovine serum albumin-coated MNPs, which presented the highest circulation time among the MNPs studied. These data showed better biocompatibility, stability and magnetic signal uniformity in biological media for bovine serum albumin-coated MNPs than for citrate-coated MNPs and bare MNPs.