Knockdown times in a simple assay determination of insecticide resistance in malaria vectors.

Trans R Soc Trop Med Hyg

Mozambican-Danish Rural Malaria Initiative (MOZDAN), P.O. Box 8, Morrumbene, Inhambane Province, Mozambique.

Published: November 2021

Background: Determining the insecticide resistance status of malaria vectors, particularly to insecticides used on mosquito nets, is important but is limited to a relatively small number of locations. We describe a simple assay that enables this information to be obtained over a much wider area.

Methods: The time to knockdown of mosquitoes in an insecticide-treated netting-covered metal frame cage were recorded. The shape of the curve of the proportion of knocked down mosquitoes provides information on resistance status.

Results: Resistant Anopheles funestus took significantly longer for knockdown than did susceptible Anopheles arabiensis.

Conclusions: This simple technique will enable a wide range of locations to be sampled, enhancing our understanding of the spread of resistance to pyrethroids.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560632PMC
http://dx.doi.org/10.1093/trstmh/trab140DOI Listing

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