Quantitative Transformation Efficiency Assay for .

Bio Protoc

Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Published: December 2018

is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of a cell which allows it to be transformed naturally. Through induction of competence, the efficiency of natural transformation can be quantified by plating colony forming units (CFU) and transforming units (TFU). Here we describe a protocol for quantifying relative transformability using .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342084PMC
http://dx.doi.org/10.21769/BioProtoc.3109DOI Listing

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