AI Article Synopsis

  • * This study investigated the impact of amantadine HCl on bovine cornea endothelial cells, finding that lower doses (≤ 20 μM) don't affect cell growth, while higher doses (≥ 50 μM) significantly inhibit growth and lead to apoptosis, DNA damage, and increased cell permeability.
  • * The results suggest that higher concentrations of amantadine HCl can disrupt cell proliferation and arrest the cell cycle, indicating its potential role in corneal edema development.

Article Abstract

Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson's disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445916PMC
http://dx.doi.org/10.1038/s41598-021-98005-9DOI Listing

Publication Analysis

Top Keywords

amantadine hcl
28
cornea endothelial
20
endothelial cells
20
bovine cornea
16
cell growth
12
doses amantadine
12
amantadine hydrochloride
8
amantadine
8
hcl
8
corneal edema
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!