Background: Live yeast Saccharomyces cerevisiae has been used for a long time in ruminant feed as a probiotic supply on the intensifying system to avoid acidosis.
Objectives: This study investigated the effects of addition of live yeast S. cerevisiae in calf feed on growth, rumen pH and in vitro digestibility.
Methods: Sixteen Holstein calves were divided into two homogeneous groups corresponding to body weight. The ration comprises wheat straw 5 kg dry matter (DM)/calf/day and 8 kg DM/calf/day concentrate for the control group C and for the group LY. Each calf of the live yeast group LY gets more than C group, 28 g/calf/day of live yeast S. cerevisiae powder on the concentrate.
Results: This supplementation improves significantly (p < 0.003) the mean daily gain during the trial (ADG) with 400 g/calf. A notable increase (p < 0.004) was seen in final body weight gain (FWG) with 39.1 kg/calf. The live yeast supplementation decreases the feed intake and significantly (p < 0.05) the feed conversion rate (FCR) average. The live yeast S. cerevisiae as probiotic supply in ruminant feed improves the growth performance and feed efficiency in fattening calves.
Conclusions: In conclusion, we mentioned that live yeast supply induces a considerable advance in growth performance for calves.
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http://dx.doi.org/10.1002/vms3.631 | DOI Listing |
Heliyon
January 2025
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
The establishment of a feeding regimen for cladocerans is crucial in contemporary aquaculture due to their significance as nutrient-rich live feeds for various aquatic species. Three experiments were conducted to optimize the growth and reproduction rates of cladocerans ( sp. and sp.
View Article and Find Full Text PDFProtein Sci
February 2025
IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
Super-resolution microscopy has revolutionized biological imaging, enabling the visualization of structures at the nanometer length scale. Its application in live cells, however, has remained challenging. To address this, we adapted LIVE-PAINT, an approach we established in yeast, for application in live mammalian cells.
View Article and Find Full Text PDFForest Fruits Organic Honey Vinegar (FFOHV) is a spontaneously fermented (yeast) and acetified (Acetic Acid Bacteria-AAB) Miombo Woodland honey vinegar developed in Zambia. Live vinegars containing live microbial cultures are marketed for their probiotic health benefits. The correlation between a well-developed gut microbiome and human health is well studied and fermented products such as live vinegar containing AAB contribute to a healthy gut microbiome.
View Article and Find Full Text PDFSTAR Protoc
January 2025
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:
The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. We describe steps for strain construction, cell cultivation, microscopy, and image analysis.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Department of Immunobiology, University of Lausanne, Epalinges, Switzerland.
Fluorescence recovery after photobleaching (FRAP) can be employed to investigate membrane lipid mixing of vacuoles in live budding yeast cells and distinguish the fused, hemi-fused or non-fused states of these organelles under physiological conditions. Here, we describe a protocol for labeling the outer and inner leaflets of vacuoles in live cells that allow to detect hemifusion intermediates and, thus, identify components necessary for fusion pore opening.
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