A dual signal-amplified electrochemiluminescence immunosensor based on core-shell CeO-Au@Pt nanosphere for procalcitonin detection.

Mikrochim Acta

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.

Published: September 2021

A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). CeO-Au@Pt composed of sea urchin-like Au@Pt nanoparticles coated on CeO hollow nanospheres was immobilized on electrode surface to electrochemically catalyze HO to produce a large number of superoxide anion (O). The immunosensor was prepared by linking the capture antibody on immobilized CeO-Au@Pt with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au star@BSA was used to bind abundant luminol for labeling the secondary antibody (Ab). Upon the sandwich-typed immunoreactions, the O could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low HO concentration and demonstrated its high application potential in the clinical assay.

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http://dx.doi.org/10.1007/s00604-021-04988-7DOI Listing

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