Association Study and Mendelian Randomization Analysis Reveal Effects of the Genetic Interaction Between and on Wood Formation in .

MicroRNAs (miRNAs), important posttranscriptional regulators of gene expression, play a crucial role in plant growth and development. A single miRNA can regulate numerous target genes, making the determination of its function and interaction with targets challenging. We identified PtomiR403b target to , which encodes a galactosyltransferase responsible for the biosynthesis of cell wall polysaccharides. We performed an association study and epistasis and Mendelian randomization (MR) analyses to explore how the genetic interaction between and its target underlies wood formation. Single nucleotide polymorphism (SNP)-based association studies identified 25 significant associations ( < 0.01, < 0.05), and and were associated with five traits, suggesting a role for PtomiR403b and in wood formation. Epistasis analysis identified 93 significant pairwise epistatic associations with 10 wood formation traits, and 37.89% of the SNP-SNP pairs indicated interactions between and . We performed an MR analysis to demonstrate the causality of the relationships between SNPs in and wood property traits and that modulates wood formation by regulating expression of . Therefore, our findings will facilitate dissection of the functions and interactions with miRNA-targets.