The complete mitochondrial genome of and phylogenetic studies of Cyprinodontidaes.

The complete mitochondrial genome of the Cyprinodontiformes fish was studied in this study. The size of the entire mitochondrial genome was 16,878 bp, and the total length of the encoding sequence was 11,435 bp, accounting for 67.75%, encoding 3800 amino acids in total. Genome base compositions were: A was 27.30%, C was 28.30%, G was 16.17%, and T was 28.23%. ND2 and CO1 started with GTG, and other protein-coding genes started with ATG. ND1, ND2, and ND3 ended with TAG, respectively, CO2 and ND4 ended with a single T, and other PCGS ended with TAA. The ribosomal RNA lengths of 12S and 16S were 950 bp and 1690 bp, respectively. The control area (D-loop) was 1194 bp in size and ranged from 15,685 to 16,878 bp. It showed negative GC skew value (-0.2728) and negative AT skewness (-0.0168). Phylogenetic analysis showed that was most closely related to . The complete sequence of the mitochondrial genome will provide a new perspective for classification and help to draw a more complete picture of species diversity within the Cyprinodontiformes.