Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407837 | PMC |
| http://dx.doi.org/10.1364/BOE.424016 | DOI Listing |
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