The enzyme phosphoribosyl pyrophosphate synthase (PRPS) catalyzes the conversion of ribose 5-phosphate into phosphoribosyl diphosphate; the latter is a precursor of purine and pyrimidine nucleotides. Here, we investigated the function of PRPS from the single-celled green alga in its response to DNA damage from gamma radiation or the alkylating agent LiCl. transcripts were upregulated in cells treated with these agents. We generated -overexpressing transgenic lines to study the function of CrPRPS. When grown in culture with LiCl or exposed to gamma radiation, the transgenic cells grew faster and had a greater survival rate than wild-type cells. overexpression enhanced expression of genes associated with DNA damage response, namely , , and We observed, from transcriptome analysis, upregulation of genes that code for key enzymes in purine metabolism, namely , , and . We conclude that may affect DNA repair process via regulation of nucleotide synthesis.
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| http://dx.doi.org/10.3389/fpls.2021.719846 | DOI Listing |
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