Antibacterial photodynamic therapy is a promising treatment for problematic infections caused by bacteria and fungi. Despite its undoubted effectiveness, the ultrastructural mechanism of microbial death remains not fully described and distinct organisms respond to the treatment with different efficacy. For this reason, it was decided to try imaging the process using the in situ transmission electron microscopy method. To conduct an observational experiment, the microscope was significantly modified. Liquid cell methods were used, electron doses and their influence on the sample were estimated, and a fiber-optic sample illuminator was designed and built. The modifications allowed for the light-induced characterization of photosensitizer-bacteria interaction. Microscope modification is a promising platform for further studies of light-induced phenomena in both life and material science.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ultramic.2021.113388 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Implementation of simultaneous ultraviolet/visible and x-ray absorption spectroscopy with microfluidics.
Rev Sci Instrum
January 2025
Max Planck Institute for Chemical Energy Conversion, Mülheim an der Ruhr 45470, Germany.
X-ray spectroscopies are uniquely poised to describe the geometric and electronic structure of metalloenzyme active sites under a wide variety of sample conditions. UV/Vis (ultraviolet/visible) spectroscopy is a similarly well-established technique that can identify and quantify catalytic intermediates. The work described here reports the first simultaneous collection of full in situ UV/Vis and high-energy resolution fluorescence detected x-ray absorption spectra.
View Article and Find Full Text PDFHigh sensitivity tapered fiber refractive index biosensor using hollow gold nanoparticles.
Sci Rep
January 2025
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
A localized surface plasmon resonance (LSPR) sensor based on tapered optical fiber (TOF) using hollow gold nanoparticles (HAuNPs) for measuring the refractive index (RI) is presented. This optical fiber sensor is a good candidate for a label-free RI biosensor. In practical biosensors, bioreceptors are immobilized on nanoparticles (NPs) that only absorb specific biomolecules.
View Article and Find Full Text PDFA Quantitative Chemometric Study of Pharmaceutical Tablet Formulations Using Multi-Spectroscopic Fibre Optic Probes.
Pharmaceuticals (Basel)
December 2024
College of Science and Engineering, Flinders University, Bedford Park, South Australia 5042, Australia.
Two fibre optic probes were custom designed to perform Raman and near-infrared spectroscopic measurements. Our long-term objective is to develop a non-destructive tool able to collect data in hard-to-access locations for real-time analysis or diagnostic purposes. This study evaluated the quantitative performances of Probe A and Probe B using model pharmaceutical tablets.
View Article and Find Full Text PDFColon Tumor Discrimination Combining Independent Endoscopic Probe-Based Raman Spectroscopy and Optical Coherence Tomography Modalities with Bayes Rule.
Int J Mol Sci
December 2024
Leibniz-Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Str. 9, 07745 Jena, Germany.
Colorectal cancer is one of the most prevalent forms of cancer globally. The most common routine diagnostic methods are the examination of the interior of the colon during colonoscopy or sigmoidoscopy, which frequently includes the removal of a biopsy sample. Optical methods, such as Raman spectroscopy (RS) and optical coherence tomography (OCT), can help to improve diagnostics and reduce the number of unnecessary biopsies.
View Article and Find Full Text PDFCompartmental analysis of retinal vascular parameters and thickness in myopic eyes using SS-OCTA.
Front Med (Lausanne)
December 2024
Chongqing Key Laboratory of Prevention and Treatment on Major Blinding Diseases, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Article Synopsis
- The study examined how myopia affects the thickness and structure of retinal blood vessels using advanced imaging technology in 100 participants.
- The findings revealed that individuals with high myopia had significantly lower vessel density and thinner retinal layers, particularly noticeable in the superficial and nerve fiber layers.
- There was a noted correlation between certain ocular parameters, such as axial length to curvature radius ratio, and retinal thickness in non-high myopic eyes, while high myopic eyes showed less correlation in these aspects.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!