[Study on emergency metabolic changes of larvae following exposure to deltamethrin].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi

Jiangsu Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Training on Malaria Elimination, Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Wuxi 214064, China.

Published: August 2021

Objective: To analyze the changes of small molecular metabolites in the larvae of a deltamethrin-sensitive strain of following exposure to deltamethrin, so as to provide the scientific basis for investigating the metabolic pathway and screening metabolic markers of deltamethrin in .

Methods: The 50% and 75% lethal concentrations (LC and LC) of deltamethrin against the larvae of a deltamethrin-sensitive strain of were calculated in laboratory. The type and content of larvae metabolites were detected using high performance liquid chromatography and mass spectrometry (LC-MS/MS) following exposure to deltamethrin at LC and LC for 30 min and 24 h, and the changes of metabolites were analyzed.

Results: The LC and LC values of deltamethrin were 4.36 × 10 µg/mL and 1.12 × 10 µg/mL against thelarvae of a deltamethrin-sensitive strain of . Following exposure of the larvae of a deltamethrin-sensitive strain of to deltamethrin at LC and LC for 30 min, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, fatty acyl and pyrimidine nucleotides, with reduced glucose levels. Following exposure for 24 h, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, aliphatic acyl and purine nucleotides, with increased glucose level detected.

Conclusions: Carbohydrate, carboxylic acid and its derivatives, fatty acyls, amino acids and their derivatives may play important roles in deltamethrin metabolism in the larvae of a deltamethrin-sensitive strain of .

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http://dx.doi.org/10.16250/j.32.1374.2020329DOI Listing

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