Objective: To analyze the changes of small molecular metabolites in the larvae of a deltamethrin-sensitive strain of following exposure to deltamethrin, so as to provide the scientific basis for investigating the metabolic pathway and screening metabolic markers of deltamethrin in .
Methods: The 50% and 75% lethal concentrations (LC and LC) of deltamethrin against the larvae of a deltamethrin-sensitive strain of were calculated in laboratory. The type and content of larvae metabolites were detected using high performance liquid chromatography and mass spectrometry (LC-MS/MS) following exposure to deltamethrin at LC and LC for 30 min and 24 h, and the changes of metabolites were analyzed.
Results: The LC and LC values of deltamethrin were 4.36 × 10 µg/mL and 1.12 × 10 µg/mL against thelarvae of a deltamethrin-sensitive strain of . Following exposure of the larvae of a deltamethrin-sensitive strain of to deltamethrin at LC and LC for 30 min, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, fatty acyl and pyrimidine nucleotides, with reduced glucose levels. Following exposure for 24 h, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, aliphatic acyl and purine nucleotides, with increased glucose level detected.
Conclusions: Carbohydrate, carboxylic acid and its derivatives, fatty acyls, amino acids and their derivatives may play important roles in deltamethrin metabolism in the larvae of a deltamethrin-sensitive strain of .
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http://dx.doi.org/10.16250/j.32.1374.2020329 | DOI Listing |
J Agric Food Chem
December 2024
Department of Pathogen Biology, Nanjing Medical University, Nanjing 211100, China.
Mosquitoes within the complex play a crucial role in human disease transmission. Insecticides, especially pyrethroids, are used to control these vectors. Mosquito legs are the main entry point and barrier for insecticides to gain their neuronal targets.
View Article and Find Full Text PDFParasit Vectors
August 2024
Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, 571199, China.
Background: Aedes albopictus is an important vector for pathogens such as dengue, Zika, and chikungunya viruses. While insecticides is the mainstay for mosquito control, their widespread and excessive use has led to the increased resistance in Ae. albopictus globally.
View Article and Find Full Text PDFParasit Vectors
October 2021
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Background: The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated.
Methods: Quantitative real-time reverse transcription PCR was used to detect the expression levels of miR-279-3p and CYP325BB1.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
August 2021
Jiangsu Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Training on Malaria Elimination, Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Wuxi 214064, China.
Objective: To analyze the changes of small molecular metabolites in the larvae of a deltamethrin-sensitive strain of following exposure to deltamethrin, so as to provide the scientific basis for investigating the metabolic pathway and screening metabolic markers of deltamethrin in .
Methods: The 50% and 75% lethal concentrations (LC and LC) of deltamethrin against the larvae of a deltamethrin-sensitive strain of were calculated in laboratory. The type and content of larvae metabolites were detected using high performance liquid chromatography and mass spectrometry (LC-MS/MS) following exposure to deltamethrin at LC and LC for 30 min and 24 h, and the changes of metabolites were analyzed.
Gene
February 2019
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. Electronic address:
Pests have been subjected to heavy selection by insecticide pressure, producing greater and more serious resistance. It is extremely valuable to identify the resistance genes that are relevant for pest control. In our previous studies, we reported that UBL40 is the deltamethrin resistance-associated gene.
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