Effects of acetylphenylhydrazine (APHZ) on the stromal cells of the bone marrow in rats (in vitro).

The study has been carried out on Wistar rats. The animals were divided into two groups: the control rats received 0.9% saline solution intraperitoneally in there consecutive days. The experimental animals were administered 1% solution of acetylphenylhydrazine (APHZ) intraperitoneally also in three days. Prior to the experiments samples of blood have been collected from tail veins and reticulocyte counts made: the counts had also been determined before the culture of tissue was started. On the fifth day of the primary culture, the morphology and enzymatic activity of the stromal cells of the bone marrow were studied. The cells grown in vitro were cultured by the use of the Eagle's medium enriched with 20% calf serum and L-glutamate. The type of the cells, their number and rate of growth were noted. In the cells the activity of specific esterase as well as acid and alkaline phosphatase was determined. Statistically significant differences between the control and experimental animals were found both in the numbers of stromal cells and in the differences in score values of activity of particular enzymes in various types of cells.