Background: To investigate the effects of small incision lenticule extraction (SMILE)-derived decellularized lenticules on intraocular pressure (IOP) and conjunctival scarring in a rabbit model of glaucoma filtration surgery.
Methods: Trabeculectomy was performed on both eyes of New Zealand rabbits. A decellularized lenticule was placed in the subconjunctival space in one eye of the rabbits (the decellularized lenticule group), and no adjunctive treatment was performed in the fellow eye (the control group). The filtering bleb features and IOP were evaluated 0, 3, 7, 14, 21, and 28 days after surgery, and histopathologic examination was performed 28 days after surgery.
Results: Decellularized lenticules significantly increased bleb survival and decreased IOP postoperatively in the rabbit model with no adverse side effects. The histopathologic results showed a larger subconjunctival space and less subconjunctival fibrosis in the decellularized lenticule group.
Conclusions: Decellularized lenticules can prevent postoperative conjunctiva-sclera adhesion and fibrosis, and they may represent a novel antifibrotic agent for trabeculectomy.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431885 | PMC |
| http://dx.doi.org/10.1186/s12886-021-02090-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SMILE-Derived Corneal Stromal Lenticule: Experimental Study as a Corneal Repair Material and Drug Carrier.
Cornea
January 2025
Chongqing Eye and Vision Care Hospital Aier Eye Hospital Group, Yu Zhong, Chongqing, China.
Purpose: A detailed study of the physicochemical properties of SMILE-derived lenticules and evaluation of their drug delivery after loading with silver nanoparticles (AgNPs).
Methods: The lenticules were decellularized and modified with crosslinking concentrations of 0.01 (0.
Xenographic lenticule implantation followed by riboflavin and UV treatment: A promising alternative for corneal ectasias management.
Methods
January 2025
Faculty of Pharmacy, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address:
The cornea is the primary refracting surface of the eye, requiring precise curvature to ensure optimal vision. Any distortion in its shape may result in significant visual impairment. Corneal ectasias, such as keratoconus (KC), is characterized by gradual thinning and protrusion of the thinned area, due to biomechanical weakening of the tissue, leading to astigmatism and vision loss.
View Article and Find Full Text PDFComparative study of physicochemical properties on corneal stromal lenticules following four decellularization methods.
Exp Eye Res
December 2024
Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, 200031, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, 200031, China; Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care, Shanghai, 200031, China. Electronic address:
This study compares the physicochemical properties of corneal stromal lenticules following decellularization via four methods. Human corneal stromal lenticules, derived from small incision lenticule extraction surgery, underwent decellularization with sodium dodecyl sulfate (SDS), Triton X-100 (Tx) combined with SDS, trypsin-ethylenediaminetetraacetic acid (TE), or NaCl combined with deoxyribonuclease (DNase), respectively. Lenticule DNA and glycosaminoglycan (GAG) content, immunofluorescence staining of cell nuclei and collagen, transparency, biomechanics, histological structure, and immunogenicity were examined in each group and compared with fresh lenticules.
View Article and Find Full Text PDFEffects of mesenchymal stromal cells and human recombinant Nerve Growth Factor delivered by bioengineered human corneal lenticule on an innovative model of diabetic retinopathy.
Front Endocrinol (Lausanne)
October 2024
Department of Medical, Oral and Biotechnological Sciences, Center for Advanced Studies and Technology-CAST, University G. d'Annunzio of Chieti-Pescara, Chieti, Italy.
Introduction: Diabetic retinopathy (DR) is a microvascular complication of diabetes in which neurodegeneration has been recently identified as a driving force. In the last years, mesenchymal stromal cells (MSCs) and neurotrophins like Nerve Growth Factor (NGF), have garnered significant attention as innovative therapeutic approaches targeting DR-associated neurodegeneration. However, delivering neurotrophic factors directly in the eye remains a challenge.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to evaluate a new desiccation method for preserving human SMILE lenticules and testing their integration in rabbit eyes.
- After implantation, the analysis showed that the lenticules maintained their structure and did not provoke any adverse reactions in the rabbit corneas over a period of 6 to 24 weeks.
- The results suggest that desiccated lenticules can integrate well in rabbit corneas, indicating potential for future clinical trials in treating conditions like hyperopia or keratoconus in humans.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!