Antagonistic Potential of Soil Isolates from Southern Thailand to Inhibit Foodborne Bacterial Pathogens.

Int J Microbiol

Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, Thailand.

Published: August 2021

are well known for their competence to produce thousands of bioactive secondary metabolites and enzymes. This study aimed to assess the inhibitory activities of crude extracts from diverse collected from rice soils in Narathiwat, Thailand, against foodborne bacterial pathogens. In total, 136 isolates were screened using a cross-streak method for the ability to produce effective metabolites against 5 pathogenic bacteria. Out of these, 19 (13.97%) isolates had antibacterial activity against at least one tested bacterium. Most of the isolates could strongly suppress the growth of ATCC25923 and MTCC430 except ATCC27853. On the basis of morphological, cultural, and biochemical characteristics, all potent isolates exhibited typical features that fitted the genus . Two of the 7 selected ethyl acetate crude extracts had good antagonistic activity against ATCC25923 and MTCC430 when tested using the agar well diffusion assay. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the 2 extracts evaluated using the colorimetric broth microdilution method ranged from 256 to >1,024 g/ml against the tested bacteria. The partial nucleotide sequences of the gene led to identifying both active isolates as species. These active isolates could provide an interesting source for generating innumerable natural compounds with antibacterial activity that can presumably be developed to fight bacterial pathogens in the near future.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421186PMC
http://dx.doi.org/10.1155/2021/2545441DOI Listing

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