Modified ELISA for antibody avidity evaluation: The need for standardization.

Biomed J

Adolfo Lutz Institute, Immunology Division, Sao Paulo, Brazil; Sao Paulo University, Biomedical Sciences Institute, Post-Graduation Program Interunity in Biotechnology, Sao Paulo, Brazil. Electronic address:

Published: August 2021

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Article Abstract

Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515003PMC
http://dx.doi.org/10.1016/j.bj.2020.10.009DOI Listing

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