Sludge lysate is an unavoidable and refractory liquid produced from the waste activated sludge hydrothermal pyrolysis, which contains plenty of hazardous refractory organic compounds and value-added organic resources. Here, the proof of concept for an integrated strategy that couples technically compatible pretreatment to microbial electrolysis assisted AD (ME-AD) system is investigated for sludge lysate treatment and resource recovery. The pretreatment process shows a positive effectiveness on the ME-AD by reducing the organic load and inhibitory matters, which promote the residual refractory organic compounds (Maillard reaction products and humic acid-like substances) and carbon sources further biodegradation and bio-transformation. Combining membrane separation with ME-AD increased not only both the yield and purity of methane to 268.76 mL CH/g COD and 98%, respectively, but also the recovery of 70.0~82.4% crude proteins (9.1 ± 0.5 g/L) from sludge lysate. Alternatively, the alkaline precipitation combined with ME-AD enhanced the recovery efficiency of short-chain fatty acids (SCFAs). The visible decreasing in the unpleasant color of the effluents was observed, implying that the degradation of harmful refractory organic was almost eliminated in sludge lysate. This strategy is worthy to be developed in WWTP for sludge lysate treatment with considerable bio-resources recovery and refractory organics removal.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126206 | DOI Listing |
Methods Mol Biol
June 2024
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
Bacteriophages (phages) are viruses that infect bacteria and are the most abundant biological entity on the planet. Phages have gained popularity as an alternative to antibiotics due to their specificity and ability to efficiently lyse antimicrobial resistant bacterial pathogens. Before using phages, they must be isolated from the environment and tested to ensure purity and lytic ability against various hosts.
View Article and Find Full Text PDFWater Res
April 2024
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. Electronic address:
Two iron-electrolysis assisted anammox/denitrification (EAD) systems, including the suspended sludge reactor (ESR) and biofilm reactor (EMR) were constructed for mainstream wastewater treatment, achieving 84.51±4.38 % and 87.
View Article and Find Full Text PDFJ Environ Manage
January 2024
Solid Waste Treatment and Resource Recycle Research Laboratory, Department of Environmental Science and Technology, School of Energy and Environment, Southeast University, Nanjing, 210096, PR China.
Sludge reduction is a critical challenge in biological wastewater treatment. Combining excess sludge slurry lysis technology with traditional activated sludge processes is a promising approach for in-situ sludge reduction. Here, a strategy for excess sludge slurry lysis based on thermophilic bacterial communities (LTBC) was proposed.
View Article and Find Full Text PDFJ Environ Manage
November 2023
Solid Waste Treatment and Resource Recycle Research Laboratory, Department of Environmental Science and Technology, School of Energy and Environment, Southeast University, Nanjing, 210096, People's Republic of China.
Sludge reduction is a major challenge in biological wastewater treatment. Hydrolytic enzymes secreted by thermophilic bacteria can lyse sludge and thus achieve sludge reduction, and the indigenous thermophilic community in sludge can lyse sludge more effectively. In this study, the feasibility of combining a sludge lysis reactor based on thermophilic bacteria community (LTBC reactor, 75 °C) with a conventional sequencing batch activated sludge reactor (SBR) for sludge reduction (i.
View Article and Find Full Text PDFEnviron Int
September 2023
Guangdong Laboratory for Lingnan Modern Agriculture, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics, Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address:
The widespread of tetracycline (TC) residues in anthropogenic and natural environments pose an immediate threat to public health. Herein, we established the TET-Yeasate, an approach based on whole-cell lysate of engineered yeast, to mitigate the TC contamination in environment. The TET-Yeasate is defined as the biological matrix of whole cell lysate from engineered yeast that containing TC-degradative components (Tet(X), NADPH, Mg) and protective macromolecules.
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