Identification of Two New Sequences of Flagellin-Encoding Gene in Using PCR and Sequencing-Based Methods.

has traditionally been serotyped using antisera against the O and H antigens. However, a proportion of isolates are nonmotile and, in addition, some isolates do not react with the currently available H-typing sera. Alternative molecular methods have been developed based on the detection of genes encoding for H antigens. In this study, we studied 13 serologically nontypable H antigen strains using polymerase chain reaction (PCR) and sequencing-based methods. We found two new sequences of flagellin-encoding gene, for each of which a specific antiserum was produced to confirm their expression. Sequencing of the flagellin gene offers a rapid determination of H antigens and could be used to detect potential novel flagellar antigens.