Clinical follow-up aided by changes in the expression of circulating microRNAs (miRs) may improve prognostication of pancreatic ductal adenocarcinoma (PDAC) patients. Changes in 179 circulating miRs due to cancer progression in the transgenic ; ; (KPC) animal model of PDAC were analyzed for serum miRs that are altered in metastatic disease. In addition, expression levels of 250 miRs were profiled before and after pancreaticoduodenectomy in the serum of two patients with resectable PDAC with different progression free survival (PFS) and analyzed for changes indicative of PDAC recurrence after resection. Three miRs that were upregulated ≥3-fold in progressive PDAC in both mice and patients were selected for validation in 26 additional PDAC patients before and after resection. We found that high serum miR-125b-5p and miR-99a-5p levels after resection are significantly associated with shorter PFS (HR 1.34 and HR 1.73 respectively). In situ hybridization for miR detection in the paired resected human PDAC tissues showed that miR-125b-5p and miR-99a-5p are highly expressed in inflammatory cells in the tumor stroma, located in clusters of CD79A expressing cells of the B-lymphocyte lineage. In conclusion, we found that circulating miR-125b-5p and miR-99a-5p are potential immune-cell related prognostic biomarkers in PDAC patients after surgery.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415800 | PMC |
| http://dx.doi.org/10.3390/app9224784 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Altered expression profile of plasma exosomal microRNAs in exclusive electronic cigarette adult users.
Sci Rep
January 2025
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, US.
Little is known about how exclusive e-cigarette use affects exosomal microRNA (miRNA) expression, which is crucial in inflammation and disease processes like cancer. We compared exosomal miRNA profiles between exclusive e-cigarette users and non-users. We used plasma samples from 15 exclusive e-cigarette users and 15 non-users from the Population Assessment of Tobacco and Health (PATH) Wave 1 study (2013-2014) and sequenced miRNAs with Illumina NextSeq 500/550.
View Article and Find Full Text PDFHighly Sensitive and Specific Panels of Plasma Exosomal microRNAs for Identification of Malignant Pulmonary Nodules.
Clin Respir J
November 2024
Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui Province, China.
Article Synopsis
- The study focuses on the use of plasma exosomal microRNAs as potential biomarkers for identifying malignant solitary pulmonary nodules (SPNs), which are often detected via CT scans.
- Researchers identified exosomal miRNAs from patients with confirmed SPNs and selected candidate miRNAs for further testing, ultimately determining diagnostic values through quantitative PCR and AUROC analysis.
- Results showed that the combination of microRNAs produced high sensitivity and specificity rates for malignant SPNs, particularly a combination of miR-1-3p and miR-99a-5p, which achieved 100% for both metrics in detection.
Article Synopsis
- MicroRNAs (miRNAs) such as miR-99a-5p, miR-100-5p, and miR-125b-5p are typically downregulated in malignant germ cell tumors (GCTs), leading to potential issues in tumor growth and behavior.
- In this study, researchers used quantitative RT-PCR and treatments like 5-azacytidine to investigate the dysregulation of these miRNAs and their effects on cancer cell growth in different types of malignant GCTs.
- They found that replenishing levels of miR-100-5p and miR-125b-5p inhibited the growth of GCT cells and altered related signaling pathways, suggesting a potential therapeutic approach
Identification of exosomal microRNAs and related hub genes associated with imatinib resistance in chronic myeloid leukemia.
Naunyn Schmiedebergs Arch Pharmacol
December 2024
Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
Chemotherapy resistance is a major obstacle in cancer therapy, and identifying novel druggable targets to reverse this phenomenon is essential. The exosome-mediated transmittance of drug resistance has been shown in various cancer models including ovarian and prostate cancer models. In this study, we aimed to investigate the role of exosomal miRNA transfer in chronic myeloid leukemia drug resistance.
View Article and Find Full Text PDFCirculating microRNAs and therapy-associated cardiac events in HER2-positive breast cancer patients: an exploratory analysis from NeoALTTO.
Breast Cancer Res Treat
July 2024
Department of Advanced Diagnostics, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
Purpose: The relevance of cardiotoxicity in the context of HER2-positive breast cancer is likely to increase with increasing patient treatment exposure, number of treatment lines, and prolonged survival. Circulating biomarkers to early identify patients at risk of cardiotoxicity could allow personalized treatment and follow-up measures. The aim of this study is to examine the relationship between circulating microRNAs and adverse cardiac events in HER2-positive breast cancer patients.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!