After many years of the excessive use of praziquantel against Schistosoma mansoni (S. mansoni), it has already led to the development of drug resistance. While schistosomiasis is still affecting millions of people every year, vaccination may be one realistic alternative way to control the disease. Currently, S. mansoni 14-kDa fatty acid-binding protein (Sm14) has shown promising results as a vaccine antigen. Yet, the use of an adjuvant may be necessary to further increase the effectiveness of the vaccine. Herein, we investigated the potential of using heat-killed Cutibacterium acnes (C. acnes) as an adjuvant for recombinant Sm14 (rSm14). Immunization of mice with C. acnes-adjuvanted rSm14 showed increased humoral immune responses, compared with mice immunized with rSm14 alone. Additionally, C. acnes-adjuvanted rSm14 vaccination provided higher protection to mice against S. mansoni infection and liver injuries. These results suggest that C. acnes increases the immunogenicity of rSm14, which leads to better protection against S. mansoni infection. Therefore, heat-killed C. acnes may be a promising adjuvant to use with rSm14.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.parint.2021.102446 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Schistosoma mansoni egg-derived thioredoxin and Sm14 drive the development of IL-10 producing regulatory B cells.
PLoS Negl Trop Dis
June 2023
Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
During chronic schistosome infections, a complex regulatory network is induced to regulate the host immune system, in which IL-10-producing regulatory B (Breg) cells play a significant role. Schistosoma mansoni soluble egg antigens (SEA) are bound and internalized by B cells and induce both human and mouse IL-10 producing Breg cells. To identify Breg-inducing proteins in SEA, we fractionated SEA by size exclusion chromatography and found 6 fractions able to induce IL-10 production by B cells (out of 18) in the high, medium and low molecular weight (MW) range.
View Article and Find Full Text PDF[Gene cloning, induction, and prokaryotic expression of a Sm14-3-3 protein from Salvia miltiorrhiza].
Zhongguo Zhong Yao Za Zhi
September 2022
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Article Synopsis
- 14-3-3 proteins are crucial for plant growth and stress responses, and a specific 14-3-3 gene from the medicinal plant Salvia miltiorrhiza was identified and characterized in this study.
- The gene, which encodes a non-transmembrane protein consisting of 259 amino acids, showed a close genetic relationship with 14-3-3 proteins from several other plant species and was successfully expressed in E. coli.
- Expression analysis revealed that the 14-3-3 gene was most active in the leaves of S. miltiorrhiza and responded rapidly to drought and hormonal treatments, suggesting its important role in regulating plant responses to abiotic stresses and potential impacts on tanshin
Increased immunogenicity and protection of recombinant Sm14 antigens by heat-killed Cutibacterium acnes in BALB/c mice infected with Schistosoma mansoni.
Parasitol Int
February 2022
Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan; Institute of Medical Sciences, Tzu Chi University, Hualien 97004, Taiwan; PhD Program in Pharmacology and Toxicology, Tzu Chi University, Hualien 97004, Taiwan. Electronic address:
After many years of the excessive use of praziquantel against Schistosoma mansoni (S. mansoni), it has already led to the development of drug resistance. While schistosomiasis is still affecting millions of people every year, vaccination may be one realistic alternative way to control the disease.
View Article and Find Full Text PDFDevelopment of a conserved chimeric vaccine based on helper T-cell and CTL epitopes for induction of strong immune response against Schistosoma mansoni using immunoinformatics approaches.
Int J Biol Macromol
December 2019
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Electronic address:
Currently, three recombinant antigens based vaccines are under clinical trials against Schistosomiasis, but there is no vaccine available for prophylaxis or therapeutic. This study was conducted to construct a multi-epitope based vaccine against Schistosoma mansoni via utilizing Sm14, Sm21.7, Sm23, Sm29, Smp80, Sm-CB and SM-TSP-2 antigens.
View Article and Find Full Text PDFBuild Your Bioprocess on a Solid Strain-β-Carotene Production in Recombinant .
Front Bioeng Biotechnol
July 2019
Centro de Aromas and Sabores, DICTUC S.A., Santiago, Chile.
Robust fermentation performance of microbial cell factories is critical for successful scaling of a biotechnological process. From shake flask cultivations to industrial-scale bioreactors, consistent strain behavior is fundamental to achieve the production targets. To assert the importance of this feature, we evaluated the impact of the yeast strain design and construction method on process scalability -from shake flasks to bench-scale fed-batch fermentations- using two recombinant strains capable of producing β-carotene; SM14 and βcar1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!