AI Article Synopsis
- The study examines how cryopreservation affects the functionality of monocyte-derived dendritic cells (MDDCs) for clinical applications like DC-based vaccines.
- It found that cryopreservation does not impact the phenotype, viability, or cytokine secretion of MDDCs, indicating that frozen cells can be effectively used in immunotherapy without variability concerns from multiple blood draws.
- However, the authors caution that differences in cryopreservation techniques and assays should be considered in future research.
Article Abstract
The study of the effect of cryopreservation on the functionality of monocyte-derived dendritic cells (MDDCs) and dendritic cells (DCs) is essential for their use in different clinical applications such as DCs-based vaccines. Its full maturation and its optimal functionality are crucial for DCs based immunotherapy. In this study, we compared MDDCs derived from fresh and cryopreserved PBMCs in the aspects of phenotype and its effect on T cells at the level of proliferation and cytokine secretion. We pulsed MDDCs obtained from fresh and cryopreserved PBMCs with two different stimuli, CEF and SEA, and the expression maturation markers and cytokine secretion were analyzed. Our results showed that the cryopreservation had no effects in the phenotype of the MDDCs obtained, cell viability, maturation markers expression and/or cytokines secretion, independently whether MDDCs had been generated from fresh or cryopreserved PBMCs. Thus, this study suggests that the use of cryopreserved cells is a good method to keep the cells before use in immunotherapy, avoiding the variability within same individual due to severe blood draws. Even so, the interpretation and comparison of different results should be done considering the different cryopreservation techniques and assays, and their effects on PBMCs, specifically on MDDC and DC cells.
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| http://dx.doi.org/10.1016/j.jim.2021.113133 | DOI Listing |
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