Identification of phenolic compounds in the buds of Algerian desf. Subsp. by antioxidant activity guided fractionation.

Objectives: A bio-guided selection and identification of the most active compounds obtained from Algerian desf. Subsp.

Methods: An antioxidant activity guided fractionation was performed on buds' extract using extensive chromatographic and spectroscopic techniques. The antioxidant potentials of isolated compounds and other unpurified fractions were evaluated against DPPH radicals using TLC plates and test tubes.

Results: The results showed that all isolated compounds and fractions exhibited eminent DPPH scavenging potential. Two coumarins (7-ethoxycoumarin and 7-hydroxy-5-methoxycoumarin) and two flavonoids (3',5,7-trihydroxy-4'-methoxyflavanone and 5,6,7,4'-tetrahydroxyflavonol-3-O-rutinoside) were isolated for the first times from the titled subspecies.

Conclusions: These results confirm that the species of is far from being exhausted of active compounds, especially polyphenols.