The aim of the study was to evaluate the in vitro nematicide activity of the allspice () and oregano () essential oils (EOs) on eggs and larvae of gastrointestinal nematodes (GIN) from ruminant. The EOs were obtained by hydro-distillation of previously dehydrated plant material. In the in vitro evaluation, the egg hatching and larval mobility of and spp. of sheep and cattle, respectively, were determined. EOs at concentrations of 20.2-252.3 µg µL with serial dilutions were used, also a negative control (distilled water + Tween 20) and Thiabendazol (77 µg µL) and Levamisole (120 µg µL) as a positive control to eggs and larvae, respectively, were placed on microplates. The results were analyzed with the PROBIT procedure. PPEO showed ovicidal effect in vitro test, with lethal doses 99 (LD) of 0.56-4.19 µg µL, while oregano essential oil (OREO) was shown to be a larvicidal activity with doses of LD of 0.15-6.60 L. The results confirm that and EOs have ovicidal and larvicidal capacity against GIN in vitro test.
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| http://dx.doi.org/10.1007/s12639-021-01401-1 | DOI Listing |
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