AI Article Synopsis
- This study enhanced the printability of a bioink composed of alginate dialdehyde and gelatin by adding 9% methylcellulose, improving shape retention.
- Samples crosslinked with barium were found to be more stable and stiffer than those crosslinked with calcium, although both showed similar levels of methylcellulose release during cell culture.
- Viability tests showed that ST2 cells thrived better in barium crosslinked samples over time, while calcium crosslinked samples saw decreasing cell viability, although some cell damage occurred due to the high viscosity of the bioink.
Article Abstract
This study used methylcellulose (MC) to improve the printability of the alginate dialdehyde-gelatin (ADA-GEL) based bioink. The printability as well as the capability to maintain shape fidelity of ADA-GEL could be enhanced by the addition of 9% (w/v) MC. Moreover, the properties of the ink crosslinked with Ca and Ba were investigated. The samples crosslinked with Ba were more stable and stiffer than the Ca crosslinked samples. However, both Ca and Ba crosslinked samples exhibited a similar trend of MC release during incubation under cell culture conditions. The toxicity test indicated that both samples (crosslinked with Ca and Ba) exhibited no toxic potential. The fabrication of cell-laden constructs using the developed bioinks was evaluated. The viability of ST2 cells in Ba crosslinked samples increased while for Ca crosslinked samples, a decreased viability was observed over the incubation time. After 21 days, cell spreading in the hydrogels crosslinked with Ba occurred. However, a certain degree of cell damage was observed after incorporating the cells in the high viscosity bioink.
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| http://dx.doi.org/10.1016/j.msec.2021.112336 | DOI Listing |
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