AI Article Synopsis
- The text discusses the development of ultra-sensitive sensors for detecting molecular biomarkers, essential for diagnosing diseases and personalizing treatment, particularly highlighted during the COVID-19 pandemic.
- It presents a new electro-optofluidic chip that can directly detect SARS-CoV-2 RNA from clinical samples, achieving single-molecule sensitivity without the need for amplification.
- The device significantly increases sensing rates by over 2,000 times and can effectively operate across a wide concentration range, making it a practical solution for COVID-19 diagnosis.
Article Abstract
Ultrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal swab samples on an electro-optofluidic chip. The device relies on optically assisted delivery of target carrying microbeads to the nanopore for single RNA detection after release. A sensing rate enhancement of over 2,000x with favorable scaling towards lower concentrations is demonstrated. The combination of target specificity, chip-scale integration and rapid detection ensures the practicality of this approach for COVID-19 diagnosis over the entire clinically relevant concentration range from 10-10 copies/mL.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400458 | PMC |
| http://dx.doi.org/10.1016/j.bios.2021.113588 | DOI Listing |
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