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Establishment of transposase-assisted low-input mA sequencing technique.
J Genet Genomics
November 2021
MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:
Library Preparation Based on Transposase Assisted RNA/DNA Hybrid Co-Tagmentation for Next-Generation Sequencing of Human Noroviruses.
Viruses
January 2021
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Human noroviruses (HuNoVs) are one of the leading causes of foodborne illnesses globally. The viral genome is the most essential information for viral source tracing and viral transmission pattern monitoring. However, whole genome sequencing of HuNoVs is still challenging due to the sequence heterogeneity among different genotypes and low titer in samples.
View Article and Find Full Text PDFTn5 transposase-assisted high-efficiency transformation of filamentous fungus Phoma herbarum YS4108.
Appl Microbiol Biotechnol
October 2008
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing, People's Republic of China.
Conventionally, filamentous fungi are transformed by using conidia or protoplasts as recipients. However, induction of sporulation is difficult in some fungi, and protoplasting is an awing, frequently frustrating, and batch-dependent work. In this study, we established a simple and convenient method to prepare single cells from mycelia without enzymatic protoplasting.
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