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The Effects of Water Volume and Bacterial Concentration on the Water Filtration Assay Used in Zebrafish Health Surveillance. | LitMetric

The number of zebrafish in biomedical research has increased exponentially over the past decades, leading to pressure on the laboratory animal community to develop and refine techniques to monitor zebrafish health so that suitable stocks can be maintained for research. The water filtration assay is a promising technique in which water from a zebrafish system is filtered, and the filter analyzed by PCR. In the present report, we studied how the volume of water tested and the concentration of bacterial pathogens affected test results. To do so, we used stock solutions of 3 zebrafish pathogens: , , and . We used these stocks to create solutions with known concentrations of each pathogen, ranging between 10² and 10 Colony Forming Units (CFU) per ml. One, 2, and 3 L of each solution was filtered using positive pressure, and the filters were submitted to a commercial lab for PCR testing. Results were fit with a logistic regression model, and the probability of obtaining a positive result were calculated. Test sensitivity varied by organism, but in general, test results were positively correlated with the volume of the water filtered and with the concentration of bacteria in solution. We conclude that a positive result can be expected for at 10 CFU per mL, at 10 CFU per ml, and at 10 CFU per mL, when 3 L of solution are filtered.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628534PMC
http://dx.doi.org/10.30802/AALAS-JAALAS-21-000004DOI Listing

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