Thanks to their photophysical properties, both organic molecular fluorophores (MFs) and inorganic quantum dots (QDs) are extensively used for bioimaging applications. However, limitations such as photobleaching for the former or blinking, size, and toxicity for the latter still constitute a challenge for numerous applications. We report here that embedding MFs in graphitic carbon dots (GDs) results in fluorophores which entirely tackle this challenge. Characterized by ultranarrow, bright, and excitation-independent emission devoid of blinking and photobleaching, these hybrid-featured nanoparticles also demonstrate their unique photophysical performances at the single-nanoparticle scale, making them appealing candidates for bioimaging applications.
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http://dx.doi.org/10.1021/jacsau.1c00055 | DOI Listing |
Mini Rev Med Chem
January 2025
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126, Pisa, Italia.
Luminescent Lanthanide (III) (Ln(III)) bioprobes (LLBs) have been extensively used in the last two decades as intracellular molecular probes in bio-imaging for the efficient revelation of analytes, to signal intracellular events (enzymes/protein activity, antigen-antibody interaction), target specific organelles, and determine parameters of particular biophysical interest, to gain important insights on pathologies or diseases. The choice of using a luminescent Ln(III) coordination compound with respect to a common organic fluorophore is intimately connected to how their photophysical sensitization (antenna effect) can be finely tuned and especially triggered to respond (even quantitatively) to a certain biophysical event, condition or analyte. While there are other reviews focused on how to design chromophoric ligands for an efficient sensitization of Ln(III) ions, both in the visible and NIR region, this review is application-driven: it is a small collection of particularly interesting examples where the LLB's emissive information is acquired by imaging the emission intensity and/or the fluorescence lifetime (fluorescence lifetime imaging microscopy, FLIM).
View Article and Find Full Text PDFACS Sens
January 2025
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Circular RNAs (circRNAs), as a class of noncoding RNA molecules with a circular structure exhibit high stability and spatiotemporal-specific expression, making them ideal cancer biomarkers for liquid biopsy. Herein, a new photoelectrochemical (PEC) biosensor for a highly sensitive circRNA assay in the whole blood of lung cancer patients was designed based on CRISPR/Cas13a-programmed Cu nanoclusters (Cu NCs) and a -scheme covalent organic framework/silver sulfide (T-COF/AgS) composite. This -scheme T-COF/AgS composite accelerates electron transfer and produces an excellent initial photocurrent.
View Article and Find Full Text PDFJACS Au
January 2025
Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012, India.
The 'escape from flatland' concept has gained significant traction in modern drug discovery, emphasizing the importance of three-dimensional molecular architectures, which serve as saturated bioisosteres of benzenoids. Bicyclo[1.1.
View Article and Find Full Text PDFJACS Au
January 2025
UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal.
The mucin -glycan sialyl Tn antigen (sTn, Neu5Acα2-6GalNAcα1--Ser/Thr) is an antigen associated with different types of cancers, often linked with a higher risk of metastasis and poor prognosis. Despite efforts to develop anti-sTn antibodies with high specificity for diagnostics and immunotherapy, challenges in eliciting high-affinity antibodies for glycan structures have limited their effectiveness, leading to low titers and short protection durations. Experimental structural insights into anti-sTn antibody specificity are lacking, hindering their optimization for cancer cell recognition.
View Article and Find Full Text PDFJACS Au
January 2025
Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China.
Altermagnets, characterized by spontaneous spin-splitting without net magnetization, are challenging to realize due to their unique spin group symmetries. Two-dimensional (2D) magnetic metal-organic frameworks (MOFs), with tunable topologies and spins, offer promising platforms for achieving altermagnetism. In this study, we propose a general strategy to create 2D altermagnetic monolayers by bridging Cr with organic ligands exhibiting nonbonding molecular orbitals (NBMOs) based on the Hückel molecular orbital theory and first-principles calculations.
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