The chromosomal gene (HRS) coding for hamster histidyl-tRNA synthetase, like many other housekeeping genes, lacks many of the features associated with promoters of RNA-polymerase-II-transcribed genes. HRS transcripts have multiple start points. Using RNase protection analysis, we also identified a 300-bp exon located only 36 bp away from the 5'-most start point of the HRS transcript. This exon hybridizes to a 3.5-kb transcript which transcribes from a different strand of DNA in the 5' region of the HRS gene. This divergent 3.5-kb transcript also has multiple transcription start points. The identity and function of this 3.5-kb transcript is not known.
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http://dx.doi.org/10.1016/0378-1119(87)90198-3 | DOI Listing |
Epigenetics Chromatin
November 2024
Department of Pathology, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.
The precise spatiotemporal expression of the hematopoietic ETS transcription factor PU.1, a key determinant of hematopoietic cell fates, is tightly regulated at the chromatin level. However, how chromatin signatures are linked to this dynamic expression pattern across different blood cell lineages remains uncharacterized.
View Article and Find Full Text PDFJ Cell Mol Med
February 2024
Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, Ohio, USA.
We identified and characterized multiple cell-type selective enhancers of the CFTR gene promoter in previous work and demonstrated active looping of these elements to the promoter. Here we address the impact of genomic spacing on these enhancer:promoter interactions and on CFTR gene expression. Using CRISPR/Cas9, we generated clonal cell lines with deletions between the -35 kb airway enhancer and the CFTR promoter in the 16HBE14o airway cell line, or between the intron 1 (185 + 10 kb) intestinal enhancer and the promoter in the Caco2 intestinal cell line.
View Article and Find Full Text PDFThe precise spatio-temporal expression of the hematopoietic ETS transcription factor that determines the hematopoietic cell fates is tightly regulated at the chromatin level. However, it remains elusive as to how chromatin signatures are linked to this dynamic expression pattern across blood cell lineages. Here we performed an unbiased and in-depth analysis of the relationship between human expression, the presence of trans-acting factors, and 3D architecture at various cis-regulatory elements (CRE) proximal to the locus.
View Article and Find Full Text PDFPathol Res Pract
November 2023
School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, 302017, Jaipur, Rajasthan, India.
MEG3, a significant long non-coding RNA (lncRNA), substantially functions in diverse biological processes, particularly breast cancer (BC) development. Within the imprinting DLK-MEG3 region on human chromosomal region 14q32.3, MEG3 spans 35 kb and encompasses ten exons.
View Article and Find Full Text PDFArch Microbiol
April 2023
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Gymnema sylvestre is a tropical climber species that is widely used in traditional medicine since ages. In the present study, the transcriptome datasets of G. sylvestre available in public domain were screened for the presence of novel plant viral sequences and a putative novel virus tentatively named as Gymnema sylvestre virus 1 (GysV1) was identified.
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