Acutely ill patients presenting with conditions such as sepsis, trauma, and congestive heart failure require judicious resuscitation in order to achieve and maintain optimal circulating blood volume. Increasingly, emergency and critical care physicians are using portable ultrasound to approximate the temporal changes of the anterior-posterior (AP)-diameter of the inferior vena cava (IVC) in order to guide fluid administration or removal. This paper proposes semi-automatic active ellipse and rectangle algorithms capable of improved and quantified measurement of the AP-diameter. The proposed algorithms are compared to manual measurement and a previously published active circle model. Results demonstrate that the rectangle model outperforms both active circle and ellipse irrespective of IVC shape and closely approximates tedious expert assessment.
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http://dx.doi.org/10.3390/jimaging5010012 | DOI Listing |
J Inorg Biochem
January 2025
College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China. Electronic address:
Developing multifunctional nanomedicines represents a frontier. We have engineered a high-capacity DNA vector basing rolling circle amplification for the delivery of copper sulfide nanoparticles (CuS NPs) and doxorubicin (DOX), coupled with multivalent aptamers (MA) that precisely target tumors, culminating in a multifunctional nanoplatform (RMALCu@DOX), which combines the chemotherapy (CT)/photothermal therapy (PTT)/chemodynamic therapy (CDT). The vector (RMAL) boasts exceptional biocompatibility and incorporates multiple copy units, enabling the precise loading of numerous CuS NPs, forming RMALCu which possesses a robust photothermal effect and superior Fenton-like catalytic activity, heralding a project of minimally invasive dual-mode (PTT/CDT) therapy.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
School of Life Sciences, Soochow University, Suzhou 215123, China.
Extrachromosomal circular DNAs (eccDNAs) has been found to be widespread and functional in various organisms. However, comparative analyses of pre- and post-infection of virus are rarely known. Herein, we investigated the changes in expression patterns of eccDNA following infection with cytoplasmic polyhedrosis virus (BmCPV) and explore the role of eccDNA in viral infection.
View Article and Find Full Text PDFJ Neurodev Disord
January 2025
Rett Syndrome Research Trust, Trumbull, CT, USA.
Background: Preclinical studies and anecdotal case reports support the potential therapeutic benefit of low-dose oral ketamine as a treatment of clinical symptoms in Rett syndrome (RTT); however, no controlled studies have been conducted in RTT to evaluate safety, tolerability and efficacy.
Design: This was a sequentially initiated, dose-escalating cohort, placebo-controlled, double blind, randomized sequence, cross-over study of oral ketamine in 6-12-year-old girls with RTT to evaluate short-term safety and tolerability and explore efficacy.
Methods: Participants were randomized to either five days treatment with oral ketamine or matched placebo, followed by a nine-day wash-out period and then crossed-over to the opposite treatment.
Sheng Wu Gong Cheng Xue Bao
January 2025
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
In recent years, the bacteriophage Φ29 (Phi29) DNA polymerase has garnered increasing attention due to its high-fidelity amplification capacity at constant temperatures. To advance the industrial application of this type of isothermal polymerases, this study mined and characterized new enzymes from the microbial metagenome based on the known Phi29 DNA polymerase sequence. The results revealed that a new enzyme, Php29 DNA polymerase, was identified in the microbial metagenome with plants as the hosts.
View Article and Find Full Text PDFFood Res Int
February 2025
Food Science and Nutrition Department, University of Minnesota, 1334 Eckles Ave, Saint Paul, MN, 55108, United States. Electronic address:
There is an ever-increasing demand for novel plant proteins that are non-allergenic, nutritionally complete, adequately functional, and can be sustainably sourced. RuBisCo is a protein that fulfills these requirements and can be sourced from alfalfa (Medicago sativa). Therefore, this study investigated several techniques to adequately extract alfalfa protein.
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