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Software-Based Three-Dimensional Deconvolution Microscopy of Cytoskeletal Proteins in Cultured Fibroblast Using Open-Source Software and Open Hardware. | LitMetric

Software-Based Three-Dimensional Deconvolution Microscopy of Cytoskeletal Proteins in Cultured Fibroblast Using Open-Source Software and Open Hardware.

J Imaging

Faculty of Health Sciences, Tsukuba University of Technology, Laboratory of Human Anatomy and Cell Biology, 4-12-7 Kasuga, Tsukuba-city, Ibaraki 305-8521, Japan.

Published: November 2019

AI Article Synopsis

  • Conventional microscopy techniques struggle with blurriness due to non-focal planes, requiring a process called "deconvolution" to clarify images.
  • The author explains how to build a cost-effective deconvolution microscope using open-source software and readily available hardware, which helps overcome the blurring issue.
  • This method allows users, even those without prior experience in electronics and optics, to create decent-quality images while saving on expensive conventional equipment.

Article Abstract

As conventional fluorescence microscopy and confocal laser scanning microscopy generally produce images with blurring at the upper and lower planes along the -axis due to non-focal plane image information, the observation of biological images requires "deconvolution." Therefore, a microscope system's individual blur function (point spread function) is determined theoretically or by actual measurement of microbeads and processed mathematically to reduce noise and eliminate blurring as much as possible. Here the author describes the use of open-source software and open hardware design to build a deconvolution microscope at low cost, using readily available software and hardware. The advantage of this method is its cost-effectiveness and ability to construct a microscope system using commercially available optical components and open-source software. Although this system does not utilize expensive equipment, such as confocal and total internal reflection fluorescence microscopes, decent images can be obtained even without previous experience in electronics and optics.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321150PMC
http://dx.doi.org/10.3390/jimaging5120088DOI Listing

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