We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341802 | PMC |
| http://dx.doi.org/10.1039/d1cb00076d | DOI Listing |
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