We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of colorectal cancer cells through the surrounding stroma. This protocol has been previously published (Coulson- Thomas , 2011 ) and is described here in more detail.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376574 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2195 | DOI Listing |
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