Measurement of RNA-induced PKR Activation . | LitMetric

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Measurement of RNA-induced PKR Activation . | LitMetric

Measurement of RNA-induced PKR Activation .

Bio Protoc

Stowers Institute for Medical Research, Kansas City, MO, USA.

Published: March 2017

Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the amount and number of RNA modifications. Activation of PKR may be an underlying mechanism for the pathogenesis of human diseases. In this protocol, I describe a method for studying levels of RNA-induced PKR activation .

Download full-text PDF	Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376569	PMC
http://dx.doi.org/10.21769/BioProtoc.2178	DOI Listing

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