AI Article Synopsis
- Researchers developed a new method to isolate and study fetal progenitor cells from the digestive tract, focusing on their characteristics before they fully differentiate.
- In 2013, Mustata successfully isolated fetal intestinal progenitors that grew as spheroids, using a culture system originally meant for adult stem cells.
- The study presents a refined protocol for isolating and maintaining gastric spheroids from the distal pre-glandular stomach, highlighting their strong self-renewal abilities.
Article Abstract
Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata described the isolation of intestinal fetal progenitors growing as spheroids in the culture system initially implemented by Sato (2009) to grow adult intestinal stem cells. Noteworthy, fetal-derived spheroids have high self-renewal capacity making easy their indefinite maintenance in culture. Here, we report an adapted protocol for isolation and culture and maintenance of fetal epithelial progenitors from distal pre-glandular stomach growing as gastric spheroids (Fernandez Vallone , 2016 ).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376515 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2089 | DOI Listing |
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