AI Article Synopsis
- The tri-dimensional culture technique, first described by Sato in 2009, helps isolate and grow epithelial stem cells from various organs, starting with the adult small intestine.
- Barker built on this method in 2010 by isolating antral stem cells to create organoids that mimic the mature pyloric epithelium, aiding in the study of gastric health and disease.
- The authors adapted Barker's protocol to examine both stable and regenerating tissues, providing detailed steps for isolating and culturing antral glands and individual cells for potential further research.
Article Abstract
The tri-dimensional culture, initially described by Sato (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker (2010), who efficiently generated organoids that recapitulate the mature pyloric epithelium . This approach is suitable and promising to study gastric function in homeostasis as well as in disease. We have adapted Barker's protocol to compare homeostatic and regenerating tissues and here, we meticulously describe, step by step, the isolation and culture of antral glands as well as the isolation of single cells from antral glands that might be useful for culture after cell sorting as an example (Fernandez Vallone , 2016 ).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376483 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2088 | DOI Listing |
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