Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and function. Histones are irreversibly modified by proteolytic clipping of their tail domains. The proteolytic clipping of histone tails is continuously attracting interest of researchers in the field of chromatin biology. We can recapitulate H3-clipping by performing H3 cleavage assay. Here, we are presenting the detailed protocol to perform H3 cleavage assay.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376558 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2085 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic and Molecular Characterization of Avian Influenza A(H9N2) Viruses from Live Bird Markets (LBM) in Senegal.
Viruses
January 2025
Département de Virologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal.
Despite extensive experience with influenza surveillance in humans in Senegal, there is limited knowledge about the actual situation and genetic diversity of avian influenza viruses (AIVs) circulating in the country, hindering control measures and pandemic risk assessment. Therefore, as part of the "One Health" approach to influenza surveillance, we conducted active AIV surveillance in two live bird markets (LBMs) in Dakar to better understand the dynamics and diversity of influenza viruses in Senegal, obtain genetic profiles of circulating AIVs, and assess the risk of emergence of novel strains and their transmission to humans. Cloacal swabs from poultry and environmental samples collected weekly from the two LBMs were screened by RT-qPCR for H5, H7, and H9 AIVs.
View Article and Find Full Text PDFGenome-Wide Identification and In Silico Expression Analysis of Gene Family in (Citrus) in Response to Abiotic Stress.
Plants (Basel)
January 2025
Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat 123, Oman.
The (citrus) plant produces various phytohormones due to the significant involvement of the carotenoid cleavage oxygenase () gene family in its growth and development. genes can be divided into two main categories: (9-cis-epoxy carotenoid dioxygenase), responsible for abscisic acid (ABA) production, and (carotenoid cleavage dioxygenase), involved in pigment and strigolactone formation. To better understand the roles and positions of gene members in relation to these hormones, researchers analyzed the clementine genome.
View Article and Find Full Text PDFRole of Copper and Zinc Ions in the Hydrolytic Degradation of Neurodegeneration-Related Peptides.
Molecules
January 2025
Dipartimento di Chimica, Università di Pavia, Via Taramelli 12, 27100 Pavia, Italy.
Spontaneous cleavage reactions normally occur in vivo on amino acid peptide backbones, leading to fragmentation products that can have different physiological roles and toxicity, particularly when the substrate of the hydrolytic processes are neuronal peptides and proteins highly related to neurodegeneration. We report a hydrolytic study performed with the HPLC-MS technique at different temperatures (4 °C and 37 °C) on peptide fragments of different neuronal proteins (amyloid-β, tau, and α-synuclein) in physiological conditions in the presence of Cu and Zn ions, two metal ions found at millimolar concentrations in amyloid plaques. The coordination of these metal ions with these peptides significantly protects their backbones toward hydrolytic degradation, preserving the entire sequences over two weeks in solution, while the free peptides in the same buffer are fully fragmented after the same or even shorter incubation period.
View Article and Find Full Text PDFInvestigation of Biodegradation, Artificial Aging and Antibacterial Properties of Poly(Butylene Succinate) Biocomposites with Onion Peels and Wheat Bran.
Materials (Basel)
January 2025
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland.
The present article focuses on the characterization of the new biocomposites of poly(butylene succinate) (PBS) with fillers of plant origin such as onion peels (OP) and durum wheat bran WB () subjected to composting and artificial aging. The susceptibility to fungal growth, cytotoxicity and antibacterial properties were also examined. The biodegradation of the samples was investigated under normalized conditions simulating an intensive aerobic composting process.
View Article and Find Full Text PDFOptimization of Plasma Welding Sequence and Performance Verification for a Fork Shaft: A Comparison of Same-Direction and Reverse-Direction Welding.
Materials (Basel)
January 2025
College of Mechanical Engineering, North China University of Science and Technology, Tangshan 063210, China.
The shift fork shaft is a key component in transmissions, connecting the shift fork in order to adjust the gear engagement. This study investigates the effects of different welding sequences on deformation and residual stress during plasma welding of the shift fork shaft. A temperature-displacement coupled finite element method, using ABAQUS simulation software and a double ellipsoid heat source model, was employed for the numerical analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!