AI Article Synopsis
- Human umbilical cord mesenchymal stem cells (HUC-MSCs) are considered effective for regenerative medicine due to their unique advantages like faster growth and easier collection compared to other stem cells.
- The study demonstrated that knocking down the SOX9 gene in HUC-MSCs reduced their ability to grow, migrate, and produce important cytokines and growth factors, ultimately affecting their regenerative properties.
- The research suggests that modifying HUC-MSCs through techniques like bioengineering could enhance their effectiveness for healing and tissue repair in clinical applications.
Article Abstract
Background And Objectives: Human umbilical cord mesenchymal stem cells (HUC-MSCs) are promising candidates for cell-based therapy in regenerative medicine or other diseases due to their superior characteristics, including higher proliferation, faster self-renewal ability, lower immunogenicity, a noninvasive harvest procedure, easy expansion in vitro, and ethical access, compared with stem cells from other sources.
Methods And Results: In the present study, we knocked down the expression of SOX9 in HUC-MSCs by lentivirus interference and found that knockdown of SOX9 inhibited the proliferation and migration of HUC-MSCs and influenced the expression of cytokines (IL-6 and IL-8), growth factors (GM-CSF and VEGF) and stemness-related genes (OCT4 and SALL4). In addition, the repair effect of skin with burn injury in rats treated with HUC-MSCs transfected with sh-control was better than that rats treated with HUC-MSCs transfected with shSOX9 or PBS, and the accessory structures of the skin, including hair follicles and glands, were greater than those in the other groups. We found that knockdown of the expression of SOX9 obviously inhibited the expression of Ki67, CK14 and CK18.
Conclusions: In conclusion, this study will provide a guide for modifying HUC-MSCs by bioengineering technology in the future.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611311 | PMC |
| http://dx.doi.org/10.15283/ijsc21078 | DOI Listing |
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