Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from belonging to the GH32 family. -As32 and As32 complexed with β-d-fructose structures were determined at a resolution of 1.94 and 1.84 Å, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant As32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from , the production of 1-kestose by As32 is increased; thus, As32 can be considered as an alternative in fructan production and other industrial applications.
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