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Nanopore Sequencing Allows Recovery of High-Quality Completely Closed Genomes of All Species from Powdered Infant Formula Overnight Enrichments.
Microorganisms
November 2024
Microbial Methods Development Branch, Division of Food and Environmental Safety, Office of Applied Microbiology and Technology (OAMT), Office of Laboratory Operations and Applied Science (OLOAS), Human Foods Program, Food & Drug Administration, College Park, MD 20740, USA.
Precision metagenomic approaches using Oxford Nanopore Technology (ONT) sequencing has been shown to allow recovery of complete genomes of foodborne bacteria from overnight enrichments of agricultural waters. This study tests the applicability of a similar approach for genome recovery from powdered infant formula (PIF) overnight enrichments, where typically dominates the overall microbiome (>90%). This study aimed to test whether using ONT sequencing of overnight PIF enrichments could recover a completely closed genome for further genomic characterization.
View Article and Find Full Text PDFEnhancing Amyloid PET Quantification: MRI-Guided Super-Resolution Using Latent Diffusion Models.
Life (Basel)
December 2024
School of Computing and Augmented Intelligence, Arizona State University, Tempe, AZ 85281, USA.
Amyloid PET imaging plays a crucial role in the diagnosis and research of Alzheimer's disease (AD), allowing non-invasive detection of amyloid-β plaques in the brain. However, the low spatial resolution of PET scans limits the accurate quantification of amyloid deposition due to partial volume effects (PVE). In this study, we propose a novel approach to addressing PVE using a latent diffusion model for resolution recovery (LDM-RR) of PET imaging.
View Article and Find Full Text PDFQuantification of Minerals in Edible Mushrooms via Optimized Microwave-Assisted Digestion: Nutritional Contributions of Fe, Mg, Na, K, and Ca.
Foods
December 2024
Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Puerto Real, 11510 Cadiz, Spain.
The intake of mushrooms provides numerous beneficial properties for the correct functioning of the human body due to their rich content in carbohydrates, proteins, fibers, vitamins, and minerals. However, most of the reports are focused on the determination of bioactive compounds and only a few regarding the essential mineral content and the evaluation of the RDI. Thus, the aim of this study was to determine the mineral composition of different cultivated ( and ) and wild edible mushrooms (, , , , , and ) collected in the south of Spain and north of Morocco.
View Article and Find Full Text PDFPooled Antibiotic Susceptibility Testing Performs Within CLSI Standards for Validation When Measured Against Broth Microdilution and Disk Diffusion Antibiotic Susceptibility Testing of Cultured Isolates.
Antibiotics (Basel)
December 2024
Department of Research and Development, Pathnostics, Irvine, CA 92618, USA.
: While new methods for measuring antimicrobial susceptibility have been associated with improved patient outcomes, they should also be validated using standard protocols for error rates and other test metrics. The objective of this study was to validate a novel susceptibility assay for complicated and recurrent urinary tract infections (UTIs): pooled antibiotic susceptibility testing (P-AST). This assay was compared to broth microdilution (BMD) and disk diffusion (DD), following Clinical and Laboratory Standards Institute (CLSI) guidelines for assessment of error rates and agreement.
View Article and Find Full Text PDFApplication of X-ray diffraction with Rietveld refinement to quantify mineral composition including crystalline silica in respirable dust.
J Occup Environ Hyg
January 2025
STAMI, National Institute of Occupational Health, Oslo, Norway.
This study aimed to test the use of Rietveld refinement on respirable aerosol samples to determine the phase of respirable crystalline silica (RCS) and other minerals. The results from the Rietveld refinement were compared to an external standard method and gravimetrical measurements. Laboratory samples consisting of α-quartz, feldspar, and calcite with variable proportions and total mass loadings were made and analyzed using the NIOSH 7500 , followed by Rietveld refinement.
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