Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles.

Objectives: The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles.

Materials And Methods: Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on , , and . Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, test, and two-way ANOVA were used for statistical analyses ( = 0.05).

Results: Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly ( < 0.05). Eluted components test with and showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group ( > 0.05). Antimicrobial activity against was maintained in chitosan group up to 30 days, and decrease in colony counts was significant ( < 0.05).

Conclusion: According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on persisted for longer time (30 days) compared to the two other bacterial species (15 days).