Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper.

J Nat Prod

Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States.

Published: August 2021

AI Article Synopsis

  • Hot peppers show potential health benefits due to their anti-inflammatory, antioxidant, and antimicrobial properties, with certain species producing antimicrobial peptides (AMPs).
  • This study used bioinformatics and peptidomics to discover and characterize two new AMPs, CC-AMP1 and CC-AMP2, from the ghost pepper, finding that they are unique and effective against Gram-negative bacteria.
  • The research highlights the value of combining different methods to explore and understand the diversity of AMPs in plants.

Article Abstract

spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions were paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper ( × ). AMP prediction algorithms revealed 115 putative AMPs within the genome, of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of a pyroglutamic acid post-translational modification of CC-AMP1 and disulfide bond connectivity of both. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibited low μM antimicrobial activity against Gram-negative bacteria, including . These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to characterize plant AMP profiles.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600445PMC
http://dx.doi.org/10.1021/acs.jnatprod.1c00281DOI Listing

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