LC-MS Identification, Isolation, and Structural Elucidation of Anti-HIV Tigliane Diterpenoids from .

Structurally diverse tigliane diterpenoids have drawn significant research interest for drug discovery over many decades. Using LC-MS-guided fractionation and separation, the first phytochemical investigation on led to the isolation of eight tiglianes (-), including two new compounds, wikstrocin D () and wikstrocin E (). The new structures were elucidated based on extensive physicochemical and spectroscopic analyses. The characteristic ESIMS/MS fragmentations of tiglianes - were also summarized. Among the isolated tiglianes, three compounds (, , and ) showed the most potent anti-HIV activity, with IC values of 0.18, 3.8, and 12.8 nM, respectively.