Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Typhimurium Intracellular Survival within .

The bacterial pathogen , which causes enteritis, has a broad host range and extensive environmental longevity. In water and soil, Salmonella interacts with protozoa and multiplies inside their phagosomes. Although this relationship resembles that between and mammalian phagocytes, the interaction mechanisms and bacterial genes involved are unclear. Here, we characterized global gene expression patterns of serovar Typhimurium within at the early stage of infection by Cappable-Seq. Gene expression features of Typhimurium within were presented with downregulation of glycolysis-related, and upregulation of glyoxylate cycle-related genes. Expression of Pathogenicity Island-1 (SPI-1), chemotaxis system, and flagellar apparatus genes was upregulated. Furthermore, expression of genes mediating oxidative stress response and iron uptake was upregulated within as well as within mammalian phagocytes. Hence, global Typhimurium gene expression patterns within help better understand the molecular mechanisms of adaptation to an amoeba cell and intracellular persistence in protozoa inhabiting water and soil ecosystems.