Optimization of Bio-Foamed Concrete Brick Strength via Bacteria Based Self-Healing and Bio-Sequestration of CO.

This research aimed to optimize the compressive strength of bio-foamed concrete brick (B-FCB) via a combination of the natural sequestration of CO and the bio-reaction of enzymes. The experiments were guided by two optimization methods, namely, 2 factorial and response surface methodology (RSM). The 2 factorial analysis was carried out to screen the important factors; then, RSM analysis was performed to optimize the compressive strength of B-FCB. Four factors, namely, density (D), concentration (B), temperature (T), and CO concentration, were selectively varied during the study. The optimum compressive strength of B-FCB was 8.22 MPa, as deduced from the following conditions: 10% CO, 3 × 10 cell/mL of B, 27 °C of T and 1800 kg/m of D after 28 days. The use of in B-FCB improved the compressive strength by 35.5% compared to the foamed concrete brick (FCB) after 28 days. A microstructure analysis by scanning electronic microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction analysis (XRD) reflected the changes in chemical element levels and calcium carbonate (CaCO) precipitation in the B-FCB pores. This was due to the surface reactions of carbonic anhydrase (CA) and urease enzyme with calcium in cement and sequestered CO during the curing time.